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KMID : 0379520000160020141
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한국독성학회지
2000 Volume.16 No. 2 p.141 ~ p.146
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Study on Estrogenic Activities of Phthalate Esters Using E-screen Assay and Competitive Binding Assay
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Han Soon-Young
Han Sang-Kuk
Moon Hyun-Ju
Kim Hyoung-Sik
Lee Dong-Ha
Kim So-Hee
Kim Tae-Sung
Park Kui-Lea
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Abstract
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Phthalate esters are used extensively as a plasticizer in the manufacture of plastic products such as PVC bags and medical devices. Recently, phthalate esters have been shown to induce endocrine system mediated responses. However. only a Jew studies have been conducted for estrogenic activity of phthalate esters. In this study estrogenic activities of seven phthalate esters. butyl benzyl phthalate (BBP), di(2-ethylhexyl) phthalate (DEHP), di-n-butylphthalate (DBP), diethylphthalate (DEP), di-n-pentylphthalate (DPP), di-n-propylphthalate (DPrP) and dicyclohexylphthalate (DCHP), were examined in vitro using E-screen assay and competitive binding assay. From the E-screen assay, BBP. DEHP. DBP and DEP showed weak estrogenic activity at the concentration of 5 mutextrm{M}. The relative proliferative effect (RPE) and the relative proliferative potency (RPP) were 50~70% and 0.01%. respectively, when compared with 500 pM of 17beta-estradiol (E2). In competitive binding assay with the rat uterine estrogen receptor (ER), BBP and DEP showed weak binding potency ［(l/10^4~1/10^5 of E2］ while DEHP and DBP scarcely bound to ER. These results suggest that some phthalate esters have weak estrogenic activities in vitro.
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KEYWORD
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Phthalate esters, Estrogenic activity, Estrogen receptor (ER), E-screen assay, Competitive binding assay
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